@article{Baquero Pérez01102025,
author = {Baquero Pérez, Manuela and Laenen, Gertjan and Loïodice, Isabelle and Garnier, Mickaël and Szachnowski, Ugo and Morillon, Antonin and Ruault, Myriam and Taddei, Angela}, 
title = {Intergenic accumulation of RNA polymerase II maintains the potential for swift transcriptional restart upon release from quiescence},
volume = {35}, 
number = {10}, 
pages = {2226-2239}, 
year = {2025}, 
doi = {10.1101/gr.279874.124}, 
abstract ={Quiescent (Q) cells are seemingly inactive, developmentally arrested cells, whose universal characteristic is the ability to promptly re-enter the cell cycle upon sensing of external cues. Q cells are responsive to the environment and flexible enough to adapt to available resources. In budding yeast, quiescent nuclear features are drastically distinct from those observed in nutrient replete conditions: The nuclear volume is reduced; the telomeres relocate from the nuclear periphery to the center of the nucleus into a hypercluster; chromatin is found in a compacted, hypoacetylated state; and transcription is globally shutdown. Yet, Q cells can restart transcription within minutes of refeeding. Here, we follow the global decrease of transcription in sorted, developing Q populations and its reactivation upon release. We find that transcription and telomere clustering dynamics in and out of quiescence are independent events. We report a genome-wide redistribution of the transcription machinery as cells progress into quiescence. Although most genes are shut down, 3% of coding genes remain active. Furthermore, RNA polymerase II (RNAPII) accumulates at one-third of gene promoters. The corresponding genes are highly enriched among those showing a high level of transcription and high frequency of expression in individual cells, shortly after cells are refed, as monitored by single-cell RNA-seq. Our results point toward a role for quiescent-specific RNAPII distribution to ensure a rapid and robust transcriptional response upon return to growth.}, 
URL = {http://genome.cshlp.org/content/35/10/2226.abstract}, 
eprint = {http://genome.cshlp.org/content/35/10/2226.full.pdf+html}, 
journal = {Genome Research} 
}