RT Journal
A1 Liu, Yulong
A1 Zhai, Gang
A1 Su, Jingzhi
A1 Gong, Yulong
A1 Yang, Bingyuan
A1 Lu, Qisheng
A1 Xi, Longwei
A1 Zheng, Yutong
A1 Cao, Jingyue
A1 Liu, Haokun
A1 Jin, Junyan
A1 Zhang, Zhimin
A1 Yang, Yunxia
A1 Zhu, Xiaoming
A1 Wang, Zhongwei
A1 Gong, Gaorui
A1 Mei, Jie
A1 Yin, Zhan
A1 Gozlan, Rodolphe E.
A1 Xie, Shouqi
A1 Han, Dong
T1 The Chinese longsnout catfish genome provides novel insights into the feeding preference and corresponding metabolic strategy of carnivores
JF Genome Research 
JO Genome Research 
YR 2024 
FD July 01 
VO 34 
IS 7 
SP 981 
OP 996 
DO 10.1101/gr.278476.123 
UL http://genome.cshlp.org/content/34/7/981.abstract 
AB Fish show variation in feeding habits to adapt to complex environments. However, the genetic basis of feeding preference and the corresponding metabolic strategies that differentiate feeding habits remain elusive. Here, by comparing the whole genome of a typical carnivorous fish (Leiocassis longirostris Günther) with that of herbivorous fish, we identify 250 genes through both positive selection and rapid evolution, including taste receptor taste receptor type 1 member 3 (tas1r3) and trypsin. We demonstrate that tas1r3 is required for carnivore preference in tas1r3-deficient zebrafish and in a diet-shifted grass carp model. We confirm that trypsin correlates with the metabolic strategies of fish with distinct feeding habits. Furthermore, marked alterations in trypsin activity and metabolic profiles are accompanied by a transition of feeding preference in tas1r3-deficient zebrafish and diet-shifted grass carp. Our results reveal a conserved adaptation between feeding preference and corresponding metabolic strategies in fish, and provide novel insights into the adaptation of feeding habits over the evolution course.