RT Journal
A1 Hirth, Alexander
A1 Fatti, Edoardo
A1 Netz, Eugen
A1 Acebron, Sergio P.
A1 Papageorgiou, Dimitris
A1 Švorinić, Andrea
A1 Cruciat, Cristina-Maria
A1 Karaulanov, Emil
A1 Gopanenko, Alexandr
A1 Zhu, Tianheng
A1 Sinning, Irmgard
A1 Krijgsveld, Jeroen
A1 Kohlbacher, Oliver
A1 Niehrs, Christof
T1 DEAD box RNA helicases are pervasive protein kinase interactors and activators
JF Genome Research 
JO Genome Research 
YR 2024 
FD June 01 
VO 34 
IS 6 
SP 952 
OP 966 
DO 10.1101/gr.278264.123 
UL http://genome.cshlp.org/content/34/6/952.abstract 
AB DEAD box (DDX) RNA helicases are a large family of ATPases, many of which have unknown functions. There is emerging evidence that besides their role in RNA biology, DDX proteins may stimulate protein kinases. To investigate if protein kinase–DDX interaction is a more widespread phenomenon, we conducted three orthogonal large-scale screens, including proteomics analysis with 32 RNA helicases, protein array profiling, and kinome-wide in vitro kinase assays. We retrieved Ser/Thr protein kinases as prominent interactors of RNA helicases and report hundreds of binary interactions. We identified members of ten protein kinase families, which bind to, and are stimulated by, DDX proteins, including CDK, CK1, CK2, DYRK, MARK, NEK, PRKC, SRPK, STE7/MAP2K, and STE20/PAK family members. We identified MARK1 in all screens and validated that DDX proteins accelerate the MARK1 catalytic rate. These findings indicate pervasive interactions between protein kinases and DEAD box RNA helicases, and provide a rich resource to explore their regulatory relationships.