RT Journal
A1 Yan, Bo
A1 Wang, Duan
A1 Ettwiller, Laurence
T1 Simultaneous assessment of human genome and methylome data in a single experiment using limited deamination of methylated cytosine
JF Genome Research 
JO Genome Research 
YR 2024 
FD June 01 
VO 34 
IS 6 
SP 904 
OP 913 
DO 10.1101/gr.278294.123 
UL http://genome.cshlp.org/content/34/6/904.abstract 
AB Multiomics require concerted recording of independent information, ideally from a single experiment. In this study, we introduce RIMS-seq2, a high-throughput technique to simultaneously sequence genomes and overlay methylation information while requiring only a small modification of the experimental protocol for high-throughput DNA sequencing to include a controlled deamination step. Importantly, the rate of deamination of 5-methylcytosine is negligible and thus does not interfere with standard DNA sequencing and data processing. Thus, RIMS-seq2 libraries from whole- or targeted-genome sequencing show the same germline variation calling accuracy and sensitivity compared with standard DNA-seq. Additionally, regional methylation levels provide an accurate map of the human methylome.