TY  - JOUR
A1  - Yan, Bo
A1  - Wang, Duan
A1  - Ettwiller, Laurence
T1  - Simultaneous assessment of human genome and methylome data in a single experiment using limited deamination of methylated cytosine
Y1  - 2024/06/01 
JF  - Genome Research 
JO  - Genome Research 
SP  - 904 
EP  - 913 
DO  - 10.1101/gr.278294.123 
VL  - 34 
IS  - 6 
UR  - http://genome.cshlp.org/content/34/6/904.abstract 
N2  - Multiomics require concerted recording of independent information, ideally from a single experiment. In this study, we introduce RIMS-seq2, a high-throughput technique to simultaneously sequence genomes and overlay methylation information while requiring only a small modification of the experimental protocol for high-throughput DNA sequencing to include a controlled deamination step. Importantly, the rate of deamination of 5-methylcytosine is negligible and thus does not interfere with standard DNA sequencing and data processing. Thus, RIMS-seq2 libraries from whole- or targeted-genome sequencing show the same germline variation calling accuracy and sensitivity compared with standard DNA-seq. Additionally, regional methylation levels provide an accurate map of the human methylome. 
ER  -