@article{Yan01062024,
author = {Yan, Bo and Wang, Duan and Ettwiller, Laurence}, 
title = {Simultaneous assessment of human genome and methylome data in a single experiment using limited deamination of methylated cytosine},
volume = {34}, 
number = {6}, 
pages = {904-913}, 
year = {2024}, 
doi = {10.1101/gr.278294.123}, 
abstract ={Multiomics require concerted recording of independent information, ideally from a single experiment. In this study, we introduce RIMS-seq2, a high-throughput technique to simultaneously sequence genomes and overlay methylation information while requiring only a small modification of the experimental protocol for high-throughput DNA sequencing to include a controlled deamination step. Importantly, the rate of deamination of 5-methylcytosine is negligible and thus does not interfere with standard DNA sequencing and data processing. Thus, RIMS-seq2 libraries from whole- or targeted-genome sequencing show the same germline variation calling accuracy and sensitivity compared with standard DNA-seq. Additionally, regional methylation levels provide an accurate map of the human methylome.}, 
URL = {http://genome.cshlp.org/content/34/6/904.abstract}, 
eprint = {http://genome.cshlp.org/content/34/6/904.full.pdf+html}, 
journal = {Genome Research} 
}