TY  - JOUR
A1  - Wu, Ting-Ying
A1  - Li, Ya-Ru
A1  - Chang, Kai-Jyun
A1  - Fang, Jhen-Cheng
A1  - Urano, Daisuke
A1  - Liu, Ming-Jung
T1  - Modeling alternative translation initiation sites in plants reveals evolutionarily conserved cis-regulatory codes in eukaryotes
Y1  - 2024/02/01 
JF  - Genome Research 
JO  - Genome Research 
SP  - 272 
EP  - 285 
DO  - 10.1101/gr.278100.123 
VL  - 34 
IS  - 2 
UR  - http://genome.cshlp.org/content/34/2/272.abstract 
N2  - mRNA translation relies on identifying translation initiation sites (TISs) in mRNAs. Alternative TISs are prevalent across plant transcriptomes, but the mechanisms for their recognition are unclear. Using ribosome profiling and machine learning, we developed models for predicting alternative TISs in the tomato (Solanum lycopersicum). Distinct feature sets were predictive of AUG and nonAUG TISs in 5′ untranslated regions and coding sequences, including a novel CU-rich sequence that promoted plant TIS activity, a translational enhancer found across dicots and monocots, and humans and viruses. Our results elucidate the mechanistic and evolutionary basis of TIS recognition, whereby cis-regulatory RNA signatures affect start site selection. The TIS prediction model provides global estimates of TISs to discover neglected protein-coding genes across plant genomes. The prevalence of cis-regulatory signatures across plant species, humans, and viruses suggests their broad and critical roles in reprogramming the translational landscape. 
ER  -