RT Journal
A1 Zhang, Xiaoli
A1 Huang, Yirui
A1 Yang, Yajing
A1 Wang, Qi-En
A1 Li, Lang
T1 Advancements in prospective single-cell lineage barcoding and their applications in research
JF Genome Research 
JO Genome Research 
YR 2024 
FD December 01 
VO 34 
IS 12 
SP 2147 
OP 2162 
DO 10.1101/gr.278944.124 
UL http://genome.cshlp.org/content/34/12/2147.abstract 
AB Single-cell lineage tracing (scLT) has emerged as a powerful tool, providing unparalleled resolution to investigate cellular dynamics, fate determination, and the underlying molecular mechanisms. This review thoroughly examines the latest prospective lineage DNA barcode tracing technologies. It further highlights pivotal studies that leverage single-cell lentiviral integration barcoding technology to unravel the dynamic nature of cell lineages in both developmental biology and cancer research. Additionally, the review navigates through critical considerations for successful experimental design in lineage tracing and addresses challenges inherent in this field, including technical limitations, complexities in data analysis, and the imperative for standardization. It also outlines current gaps in knowledge and suggests future research directions, contributing to the ongoing advancement of scLT studies.