TY  - JOUR
A1  - Lyu, Lin
A1  - Li, Xue
A1  - Feng, Ru
A1  - Zhou, Xin
A1  - Guha, Tuhin K.
A1  - Yu, Xiaofei
A1  - Chen, Guo Qiang
A1  - Yao, Yufeng
A1  - Su, Bing
A1  - Zou, Duowu
A1  - Snyder, Michael P.
A1  - Chen, Lei
T1  - Simultaneous profiling of host expression and microbial abundance by spatial metatranscriptome sequencing
Y1  - 2023/03/01 
JF  - Genome Research 
JO  - Genome Research 
SP  - 401 
EP  - 411 
DO  - 10.1101/gr.277178.122 
VL  - 33 
IS  - 3 
UR  - http://genome.cshlp.org/content/33/3/401.abstract 
N2  - We developed an analysis pipeline that can extract microbial sequences from spatial transcriptomic (ST) data and assign taxonomic labels, generating a spatial microbial abundance matrix in addition to the default host expression matrix, enabling simultaneous analysis of host expression and microbial distribution. We called the pipeline spatial metatranscriptome (SMT) and applied it on both human and murine intestinal sections and validated the spatial microbial abundance information with alternative assays. Biological insights were gained from these novel data that showed host–microbe interaction at various spatial scales. Finally, we tested experimental modification that can increase microbial capture while preserving host spatial expression quality and, by use of positive controls, quantitatively showed the capture efficiency and recall of our methods. This proof-of-concept work shows the feasibility of SMT analysis and paves the way for further experimental optimization and application. 
ER  -