RT Journal
A1 Yang, Qiuyun
A1 Zhang, Lingxuan
A1 Li, Manrui
A1 Xu, Yang
A1 Chen, Xiaogang
A1 Yuan, Ruixuan
A1 Ou, Xiaofeng
A1 He, Min
A1 Liao, Miao
A1 Zhang, Lin
A1 Dai, Hao
A1 Lv, Meili
A1 Xie, Xiaoqi
A1 Liang, Weibo
A1 Chen, Xiameng
T1 Single-nucleus transcriptomic mapping uncovers targets for traumatic brain injury
JF Genome Research 
JO Genome Research 
YR 2023 
FD October 01 
VO 33 
IS 10 
SP 1818 
OP 1832 
DO 10.1101/gr.277881.123 
UL http://genome.cshlp.org/content/33/10/1818.abstract 
AB The subventricular zone (SVZ) is a neurogenic niche that contributes to homeostasis and repair after brain injury. However, the effects of mild traumatic brain injury (mTBI) on the divergence of the regulatory DNA landscape within the SVZ and its link to functional alterations remain unexplored. In this study, we mapped the transcriptome atlas of murine SVZ and its responses to mTBI at the single-cell level. We observed cell-specific gene expression changes following mTBI and unveiled diverse cell-to-cell interaction networks that influence a wide array of cellular processes. Moreover, we report novel neurogenesis lineage trajectories and related key transcription factors, which we validate through loss-of-function experiments. Specifically, we validate the role of Tcf7l1, a cell cycle gene regulator, in promoting neural stem cell differentiation toward the neuronal lineage after mTBI, providing a potential target for regenerative medicine. Overall, our study profiles an SVZ transcriptome reference map, which underlies the differential cellular behavior in response to mTBI. The identified key genes and pathways that may ameliorate brain damage or facilitate neural repair serve as a comprehensive resource for drug discovery in the context of mTBI.