RT Journal
A1 Luo, Zhengyu
A1 Zhang, Ran
A1 Hu, Tengfei
A1 Zhu, Yuting
A1 Wu, Yueming
A1 Li, Wenfei
A1 Zhang, Zhi
A1 Yao, Xuebiao
A1 Liang, Haiyi
A1 Song, Xiaoyuan
T1 NicE-C efficiently reveals open chromatin–associated chromosome interactions at high resolution
JF Genome Research 
JO Genome Research 
YR 2022 
FD March 01 
VO 32 
IS 3 
SP 534 
OP 544 
DO 10.1101/gr.275986.121 
UL http://genome.cshlp.org/content/32/3/534.abstract 
AB Enhancer–promoter communication is known to regulate spatiotemporal dynamics of gene expression. Several methods are available to capture enhancer–promoter interactions, but they either require large amounts of starting materials and are costly, or provide a relative low resolution in chromatin contact maps. Here, we present nicking enzyme-assisted open chromatin interaction capture (NicE-C), a method that leverages nicking enzyme–mediated open chromatin profiling and chromosome conformation capture to enable robust and cost-effective detection of open chromatin interactions at high resolution, especially enhancer–promoter interactions. Using TNF stimulation and mouse kidney aging as models, we applied NicE-C to reveal characteristics of dynamic enhancer–promoter interactions.