TY  - JOUR
A1  - Wang, Xiangxiu
A1  - Wang, Wen
A1  - Wang, Yiman
A1  - Chen, Jia
A1  - Liu, Guifen
A1  - Zhang, Yong
T1  - Antibody-free profiling of transcription factor occupancy during early embryogenesis by FitCUT&RUN
Y1  - 2022/02/01 
JF  - Genome Research 
JO  - Genome Research 
SP  - 378 
EP  - 388 
DO  - 10.1101/gr.275837.121 
VL  - 32 
IS  - 2 
UR  - http://genome.cshlp.org/content/32/2/378.abstract 
N2  - Key transcription factors (TFs) play critical roles in zygotic genome activation (ZGA) during early embryogenesis, whereas genome-wide occupancies of only a few factors have been profiled during ZGA due to the limitation of cell numbers or the lack of high-quality antibodies. Here, we present FitCUT&RUN, a modified CUT&RUN method, in which an Fc fragment of immunoglobulin G is used for tagging, to profile TF occupancy in an antibody-free manner and demonstrate its reliability and robustness using as few as 5000 K562 cells. We applied FitCUT&RUN to zebrafish undergoing embryogenesis to generate reliable occupancy profiles of three known activators of zebrafish ZGA: Nanog, Pou5f3, and Sox19b. By profiling the time-series occupancy of Nanog during zebrafish ZGA, we observed a clear trend toward a gradual increase in Nanog occupancy and found that Nanog occupancy prior to the major phase of ZGA is important for the activation of some early transcribed genes. 
ER  -