@article{Wang01022022,
author = {Wang, Xiangxiu and Wang, Wen and Wang, Yiman and Chen, Jia and Liu, Guifen and Zhang, Yong}, 
title = {Antibody-free profiling of transcription factor occupancy during early embryogenesis by FitCUT&RUN},
volume = {32}, 
number = {2}, 
pages = {378-388}, 
year = {2022}, 
doi = {10.1101/gr.275837.121}, 
abstract ={Key transcription factors (TFs) play critical roles in zygotic genome activation (ZGA) during early embryogenesis, whereas genome-wide occupancies of only a few factors have been profiled during ZGA due to the limitation of cell numbers or the lack of high-quality antibodies. Here, we present FitCUT&RUN, a modified CUT&RUN method, in which an Fc fragment of immunoglobulin G is used for tagging, to profile TF occupancy in an antibody-free manner and demonstrate its reliability and robustness using as few as 5000 K562 cells. We applied FitCUT&RUN to zebrafish undergoing embryogenesis to generate reliable occupancy profiles of three known activators of zebrafish ZGA: Nanog, Pou5f3, and Sox19b. By profiling the time-series occupancy of Nanog during zebrafish ZGA, we observed a clear trend toward a gradual increase in Nanog occupancy and found that Nanog occupancy prior to the major phase of ZGA is important for the activation of some early transcribed genes.}, 
URL = {http://genome.cshlp.org/content/32/2/378.abstract}, 
eprint = {http://genome.cshlp.org/content/32/2/378.full.pdf+html}, 
journal = {Genome Research} 
}