RT Journal
A1 Ham, Seokjin
A1 Kim, Sieun S.
A1 Park, Sangsoon
A1 Kim, Eun Ji E.
A1 Kwon, Sujeong
A1 Park, Hae-Eun H.
A1 Jung, Yoonji
A1 Lee, Seung-Jae V.
T1 Systematic transcriptome analysis associated with physiological and chronological aging in Caenorhabditis elegans
JF Genome Research 
JO Genome Research 
YR 2022 
FD November 01 
VO 32 
IS 11-12 
SP 2003 
OP 2014 
DO 10.1101/gr.276515.121 
UL http://genome.cshlp.org/content/32/11-12/2003.abstract 
AB Aging is associated with changes in a variety of biological processes at the transcriptomic level, including gene expression. Two types of aging occur during a lifetime: chronological and physiological aging. However, dissecting the difference between chronological and physiological ages at the transcriptomic level has been a challenge because of its complexity. We analyzed the transcriptomic features associated with physiological and chronological aging using Caenorhabditis elegans as a model. Many structural and functional transcript elements, such as noncoding RNAs and intron-derived transcripts, were up-regulated with chronological aging. In contrast, mRNAs with many biological functions, including RNA processing, were down-regulated with physiological aging. We also identified an age-dependent increase in the usage of distal 3′ splice sites in mRNA transcripts as a biomarker of physiological aging. Our study provides crucial information for dissecting chronological and physiological aging at the transcriptomic level.