RT Journal
A1 Huang, Jia
A1 Chen, Wenjun
A1 Zhou, Fan
A1 Pang, Zhichang
A1 Wang, Luoluo
A1 Pan, Tao
A1 Wang, Xiaoyun
T1 Tissue-specific reprogramming of host tRNA transcriptome by the microbiome
JF Genome Research 
JO Genome Research 
YR 2021 
FD June 01 
VO 31 
IS 6 
SP 947 
OP 957 
DO 10.1101/gr.272153.120 
UL http://genome.cshlp.org/content/31/6/947.abstract 
AB Transfer RNAs (tRNAs) are essential for translation, and tRNA expression and modifications are regulated by many factors. However, the interplay between the microbiome and host tRNA profiles through host-microbiome interactions has not been explored. In this study, we investigated host-microbiome interactions via the tRNA profiling of four tissue types from germ-free and specific pathogen-free mice. Our analyses reveal that cytosolic and mitochondrial tRNA expression and tRNA modifications in the host are reprogrammed in a tissue-specific and microbiome-dependent manner. In terms of tRNA expression, the intestines and brains are more sensitive to the influence of the microbiome than the livers and kidneys. In terms of tRNA modifications, cytosolic tRNAs show more obvious changes in the livers and kidneys in the presence of the microbiome. Our findings reveal a previously unexplored relationship among the microbiome, tRNA abundance, and epitranscriptome in a mammalian host.