RT Journal
A1 Bohm, Kaitlynne A.
A1 Hodges, Amelia J.
A1 Czaja, Wioletta
A1 Selvam, Kathiresan
A1 Smerdon, Michael J.
A1 Mao, Peng
A1 Wyrick, John J.
T1 Distinct roles for RSC and SWI/SNF chromatin remodelers in genomic excision repair
JF Genome Research 
JO Genome Research 
YR 2021 
FD June 01 
VO 31 
IS 6 
SP 1047 
OP 1059 
DO 10.1101/gr.274373.120 
UL http://genome.cshlp.org/content/31/6/1047.abstract 
AB Nucleosomes are a significant barrier to the repair of UV damage because they impede damage recognition by nucleotide excision repair (NER). The RSC and SWI/SNF chromatin remodelers function in cells to promote DNA access by moving or evicting nucleosomes, and both have been linked to NER in yeast. Here, we report genome-wide repair maps of UV-induced cyclobutane pyrimidine dimers (CPDs) in yeast cells lacking RSC or SWI/SNF activity. Our data indicate that SWI/SNF is not generally required for NER but instead promotes repair of CPD lesions at specific yeast genes. In contrast, mutation or depletion of RSC subunits causes a general defect in NER across the yeast genome. Our data indicate that RSC is required for repair not only in nucleosomal DNA but also in neighboring linker DNA and nucleosome-free regions (NFRs). Although depletion of the RSC catalytic subunit also affects base excision repair (BER) of N-methylpurine (NMP) lesions, RSC activity is less important for BER in linker DNA and NFRs. Furthermore, our data indicate that RSC plays a direct role in transcription-coupled NER (TC-NER) of transcribed DNA. These findings help to define the specific genomic and chromatin contexts in which each chromatin remodeler functions in DNA repair, and indicate that RSC plays a unique function in facilitating repair by both NER subpathways.