TY  - JOUR
A1  - Harney, Éadaoin
A1  - Cheronet, Olivia
A1  - Fernandes, Daniel M.
A1  - Sirak, Kendra
A1  - Mah, Matthew
A1  - Bernardos, Rebecca
A1  - Adamski, Nicole
A1  - Broomandkhoshbacht, Nasreen
A1  - Callan, Kimberly
A1  - Lawson, Ann Marie
A1  - Oppenheimer, Jonas
A1  - Stewardson, Kristin
A1  - Zalzala, Fatma
A1  - Anders, Alexandra
A1  - Candilio, Francesca
A1  - Constantinescu, Mihai
A1  - Coppa, Alfredo
A1  - Ciobanu, Ion
A1  - Dani, János
A1  - Gallina, Zsolt
A1  - Genchi, Francesco
A1  - Nagy, Emese Gyöngyvér
A1  - Hajdu, Tamás
A1  - Hellebrandt, Magdolna
A1  - Horváth, Antónia
A1  - Király, Ágnes
A1  - Kiss, Krisztián
A1  - Kolozsi, Barbara
A1  - Kovács, Péter
A1  - Köhler, Kitti
A1  - Lucci, Michaela
A1  - Pap, Ildikó
A1  - Popovici, Sergiu
A1  - Raczky, Pál
A1  - Simalcsik, Angela
A1  - Szeniczey, Tamás
A1  - Vasilyev, Sergey
A1  - Virag, Cristian
A1  - Rohland, Nadin
A1  - Reich, David
A1  - Pinhasi, Ron
T1  - A minimally destructive protocol for DNA extraction from ancient teeth
Y1  - 2021/03/01 
JF  - Genome Research 
JO  - Genome Research 
SP  - 472 
EP  - 483 
DO  - 10.1101/gr.267534.120 
VL  - 31 
IS  - 3 
UR  - http://genome.cshlp.org/content/31/3/472.abstract 
N2  - Ancient DNA sampling methods—although optimized for efficient DNA extraction—are destructive, relying on drilling or cutting and powdering (parts of) bones and teeth. As the field of ancient DNA has grown, so have concerns about the impact of destructive sampling of the skeletal remains from which ancient DNA is obtained. Due to a particularly high concentration of endogenous DNA, the cementum of tooth roots is often targeted for ancient DNA sampling, but destructive sampling methods of the cementum often result in the loss of at least one entire root. Here, we present a minimally destructive method for extracting ancient DNA from dental cementum present on the surface of tooth roots. This method does not require destructive drilling or grinding, and, following extraction, the tooth remains safe to handle and suitable for most morphological studies, as well as other biochemical studies, such as radiocarbon dating. We extracted and sequenced ancient DNA from 30 teeth (and nine corresponding petrous bones) using this minimally destructive extraction method in addition to a typical tooth sampling method. We find that the minimally destructive method can provide ancient DNA that is of comparable quality to extracts produced from teeth that have undergone destructive sampling processes. Further, we find that a rigorous cleaning of the tooth surface combining diluted bleach and UV light irradiation seems sufficient to minimize external contaminants usually removed through the physical removal of a superficial layer when sampling through regular powdering methods. 
ER  -