RT Journal
A1 Li, Kai
A1 Xu, Jiayue
A1 Luo, Yanyun
A1 Zou, Dingfeng
A1 Han, Ruiqin
A1 Zhong, Shunshun
A1 Zhao, Qing
A1 Mang, Xinyu
A1 Li, Mengzhen
A1 Si, Yanmin
A1 Lu, Yan
A1 Li, Pengyu
A1 Jin, Cheng
A1 Wang, Zhipeng
A1 Wang, Fang
A1 Miao, Shiying
A1 Wen, Bo
A1 Wang, Linfang
A1 Ma, Yanni
A1 Yu, Jia
A1 Song, Wei
T1 Panoramic transcriptome analysis and functional screening of long noncoding RNAs in mouse spermatogenesis
JF Genome Research 
JO Genome Research 
YR 2021 
FD January 01 
VO 31 
IS 1 
SP 13 
OP 26 
DO 10.1101/gr.264333.120 
UL http://genome.cshlp.org/content/31/1/13.abstract 
AB Long noncoding RNAs (lncRNAs) have emerged as diverse functional regulators involved in mammalian development; however, large-scale functional investigation of lncRNAs in mammalian spermatogenesis in vivo is lacking. Here, we delineated the global lncRNA expression landscape in mouse spermatogenesis and identified 968 germ cell signature lncRNAs. By combining bioinformatics and functional screening, we identified three functional lncRNAs (Gm4665, 1700027A15Rik, and 1700052I22Rik) that directly influence spermatogenesis in vivo. Knocking down Gm4665 hampered the development of round spermatids into elongating spermatids and disrupted key spermatogenic gene expression. Mechanistically, lncRNA Gm4665 localized in the nucleus of round spermatids and occupied the genomic regulatory region of important spermatogenic genes including Ip6k1 and Akap3. These findings provide a valuable resource and framework for future functional analysis of lncRNAs in spermatogenesis and their potential roles in other biological processes.