TY  - JOUR
A1  - Li, Kai
A1  - Xu, Jiayue
A1  - Luo, Yanyun
A1  - Zou, Dingfeng
A1  - Han, Ruiqin
A1  - Zhong, Shunshun
A1  - Zhao, Qing
A1  - Mang, Xinyu
A1  - Li, Mengzhen
A1  - Si, Yanmin
A1  - Lu, Yan
A1  - Li, Pengyu
A1  - Jin, Cheng
A1  - Wang, Zhipeng
A1  - Wang, Fang
A1  - Miao, Shiying
A1  - Wen, Bo
A1  - Wang, Linfang
A1  - Ma, Yanni
A1  - Yu, Jia
A1  - Song, Wei
T1  - Panoramic transcriptome analysis and functional screening of long noncoding RNAs in mouse spermatogenesis
Y1  - 2021/01/01 
JF  - Genome Research 
JO  - Genome Research 
SP  - 13 
EP  - 26 
DO  - 10.1101/gr.264333.120 
VL  - 31 
IS  - 1 
UR  - http://genome.cshlp.org/content/31/1/13.abstract 
N2  - Long noncoding RNAs (lncRNAs) have emerged as diverse functional regulators involved in mammalian development; however, large-scale functional investigation of lncRNAs in mammalian spermatogenesis in vivo is lacking. Here, we delineated the global lncRNA expression landscape in mouse spermatogenesis and identified 968 germ cell signature lncRNAs. By combining bioinformatics and functional screening, we identified three functional lncRNAs (Gm4665, 1700027A15Rik, and 1700052I22Rik) that directly influence spermatogenesis in vivo. Knocking down Gm4665 hampered the development of round spermatids into elongating spermatids and disrupted key spermatogenic gene expression. Mechanistically, lncRNA Gm4665 localized in the nucleus of round spermatids and occupied the genomic regulatory region of important spermatogenic genes including Ip6k1 and Akap3. These findings provide a valuable resource and framework for future functional analysis of lncRNAs in spermatogenesis and their potential roles in other biological processes. 
ER  -