TY  - JOUR
A1  - Chaudhary, Narendra
A1  - Nho, Si-Hyeong
A1  - Cho, Hayoon
A1  - Gantumur, Narangerel
A1  - Ra, Jae Sun
A1  - Myung, Kyungjae
A1  - Kim, Hajin
T1  - Background-suppressed live visualization of genomic loci with an improved CRISPR system based on a split fluorophore
Y1  - 2020/09/01 
JF  - Genome Research 
JO  - Genome Research 
SP  - 1306 
EP  - 1316 
DO  - 10.1101/gr.260018.119 
VL  - 30 
IS  - 9 
UR  - http://genome.cshlp.org/content/30/9/1306.abstract 
N2  - The higher-order structural organization and dynamics of the chromosomes play a central role in gene regulation. To explore this structure–function relationship, it is necessary to directly visualize genomic elements in living cells. Genome imaging based on the CRISPR system is a powerful approach but has limited applicability due to background signals and nonspecific aggregation of fluorophores within nuclei. To address this issue, we developed a novel visualization scheme combining tripartite fluorescent proteins with the SunTag system and demonstrated that it strongly suppressed background fluorescence and amplified locus-specific signals, allowing long-term tracking of genomic loci. We integrated the multicomponent CRISPR system into stable cell lines to allow quantitative and reliable analysis of dynamic behaviors of genomic loci. Due to the greatly elevated signal-to-background ratio, target loci with only small numbers of sequence repeats could be successfully tracked, even under a conventional fluorescence microscope. This feature enables the application of CRISPR-based imaging to loci throughout the genome and opens up new possibilities for the study of nuclear processes in living cells. 
ER  -