@article{Kolovos01082020,
author = {Kolovos, Petros and Nishimura, Koutarou and Sankar, Aditya and Sidoli, Simone and Cloos, Paul A. and Helin, Kristian and Christensen, Jesper}, 
title = {PR-DUB maintains the expression of critical genes through FOXK1/2- and ASXL1/2/3-dependent recruitment to chromatin and H2AK119ub1 deubiquitination},
volume = {30}, 
number = {8}, 
pages = {1119-1130}, 
year = {2020}, 
doi = {10.1101/gr.261016.120}, 
abstract ={Polycomb group proteins are important for maintaining gene expression patterns and cell identity in metazoans. The mammalian Polycomb repressive deubiquitinase (PR-DUB) complexes catalyze removal of monoubiquitination on lysine 119 of histone H2A (H2AK119ub1) through a multiprotein core comprised of BAP1, HCFC1, FOXK1/2, and OGT in combination with either of ASXL1, 2, or 3. Mutations in PR-DUB components are frequent in cancer. However, mechanistic understanding of PR-DUB function in gene regulation is limited. Here, we show that BAP1 is dependent on the ASXL proteins and FOXK1/2 in facilitating gene activation across the genome. Although PR-DUB was previously shown to cooperate with PRC2, we observed minimal overlap and functional interaction between BAP1 and PRC2 in embryonic stem cells. Collectively, these results demonstrate that PR-DUB, by counteracting accumulation of H2AK119ub1, maintains chromatin in an optimal configuration ensuring expression of genes important for general functions such as cell metabolism and homeostasis.}, 
URL = {http://genome.cshlp.org/content/30/8/1119.abstract}, 
eprint = {http://genome.cshlp.org/content/30/8/1119.full.pdf+html}, 
journal = {Genome Research} 
}