TY  - JOUR
A1  - Kapoor, Utkarsh
A1  - Licht, Konstantin
A1  - Amman, Fabian
A1  - Jakobi, Tobias
A1  - Martin, David
A1  - Dieterich, Christoph
A1  - Jantsch, Michael F.
T1  - ADAR-deficiency perturbs the global splicing landscape in mouse tissues
Y1  - 2020/08/01 
JF  - Genome Research 
JO  - Genome Research 
SP  - 1107 
EP  - 1118 
DO  - 10.1101/gr.256933.119 
VL  - 30 
IS  - 8 
UR  - http://genome.cshlp.org/content/30/8/1107.abstract 
N2  - Adenosine-to-inosine RNA editing and pre-mRNA splicing largely occur cotranscriptionally and influence each other. Here, we use mice deficient in either one of the two editing enzymes ADAR (ADAR1) or ADARB1 (ADAR2) to determine the transcriptome-wide impact of RNA editing on splicing across different tissues. We find that ADAR has a 100× higher impact on splicing than ADARB1, although both enzymes target a similar number of substrates with a large common overlap. Consistently, differentially spliced regions frequently harbor ADAR editing sites. Moreover, catalytically dead ADAR also impacts splicing, demonstrating that RNA binding of ADAR affects splicing. In contrast, ADARB1 editing sites are found enriched 5′ of differentially spliced regions. Several of these ADARB1-mediated editing events change splice consensus sequences, therefore strongly influencing splicing of some mRNAs. A significant overlap between differentially edited and differentially spliced sites suggests evolutionary selection toward splicing being regulated by editing in a tissue-specific manner. 
ER  -