TY  - JOUR
A1  - Chen, Zhoutao
A1  - Pham, Long
A1  - Wu, Tsai-Chin
A1  - Mo, Guoya
A1  - Xia, Yu
A1  - Chang, Peter L.
A1  - Porter, Devin
A1  - Phan, Tan
A1  - Che, Huu
A1  - Tran, Hao
A1  - Bansal, Vikas
A1  - Shaffer, Justin
A1  - Belda-Ferre, Pedro
A1  - Humphrey, Greg
A1  - Knight, Rob
A1  - Pevzner, Pavel
A1  - Pham, Son
A1  - Wang, Yong
A1  - Lei, Ming
T1  - Ultralow-input single-tube linked-read library method enables short-read second-generation sequencing systems to routinely generate highly accurate and economical long-range sequencing information
Y1  - 2020/06/01 
JF  - Genome Research 
JO  - Genome Research 
SP  - 898 
EP  - 909 
DO  - 10.1101/gr.260380.119 
VL  - 30 
IS  - 6 
UR  - http://genome.cshlp.org/content/30/6/898.abstract 
N2  - Long-range sequencing information is required for haplotype phasing, de novo assembly, and structural variation detection. Current long-read sequencing technologies can provide valuable long-range information but at a high cost with low accuracy and high DNA input requirements. We have developed a single-tube Transposase Enzyme Linked Long-read Sequencing (TELL-seq) technology, which enables a low-cost, high-accuracy, and high-throughput short-read second-generation sequencer to generate over 100 kb of long-range sequencing information with as little as 0.1 ng input material. In a PCR tube, millions of clonally barcoded beads are used to uniquely barcode long DNA molecules in an open bulk reaction without dilution and compartmentation. The barcoded linked-reads are used to successfully assemble genomes ranging from microbes to human. These linked-reads also generate megabase-long phased blocks and provide a cost-effective tool for detecting structural variants in a genome, which are important to identify compound heterozygosity in recessive Mendelian diseases and discover genetic drivers and diagnostic biomarkers in cancers. 
ER  -