@article{Hao01112020,
author = {Hao, Yajing and Wang, Dongpeng and Wu, Shuheng and Li, Xiao and Shao, Changwei and Zhang, Peng and Chen, Jia-Yu and Lim, Do-Hwan and Fu, Xiang-Dong and Chen, Runsheng and He, Shunmin}, 
title = {Active retrotransposons help maintain pericentromeric heterochromatin required for faithful cell division},
volume = {30}, 
number = {11}, 
pages = {1570-1582}, 
year = {2020}, 
doi = {10.1101/gr.256131.119}, 
abstract ={Retrotransposons are populated in vertebrate genomes, and when active, are thought to cause genome instability with potential benefit to genome evolution. Retrotransposon-derived RNAs are also known to give rise to small endo-siRNAs to help maintain heterochromatin at their sites of transcription; however, as not all heterochromatic regions are equally active in transcription, it remains unclear how heterochromatin is maintained across the genome. Here, we address these problems by defining the origins of repeat-derived RNAs and their specific chromatin locations in Drosophila S2 cells. We demonstrate that repeat RNAs are predominantly derived from active gypsy elements and processed by Dcr-2 into small RNAs to help maintain pericentromeric heterochromatin. We also show in cultured S2 cells that synthetic repeat-derived endo-siRNA mimics are sufficient to rescue Dcr-2-deficiency-induced defects in heterochromatin formation in interphase and chromosome segregation during mitosis, demonstrating that active retrotransposons are required for stable genetic inheritance.}, 
URL = {http://genome.cshlp.org/content/30/11/1570.abstract}, 
eprint = {http://genome.cshlp.org/content/30/11/1570.full.pdf+html}, 
journal = {Genome Research} 
}