RT Journal
A1 Sun, Yonghua
A1 Zhang, Bo
A1 Luo, Lingfei
A1 Shi, De-Li
A1 Wang, Han
A1 Cui, Zongbin
A1 Huang, Honghui
A1 Cao, Ying
A1 Shu, Xiaodong
A1 Zhang, Wenqing
A1 Zhou, Jianfeng
A1 Li, Yun
A1 Du, Jiulin
A1 Zhao, Qingshun
A1 Chen, Jun
A1 Zhong, Hanbing
A1 Zhong, Tao P.
A1 Li, Li
A1 Xiong, Jing-Wei
A1 Peng, Jinrong
A1 Xiao, Wuhan
A1 Zhang, Jian
A1 Yao, Jihua
A1 Yin, Zhan
A1 Mo, Xianming
A1 Peng, Gang
A1 Zhu, Jun
A1 Chen, Yan
A1 Zhou, Yong
A1 Liu, Dong
A1 Pan, Weijun
A1 Zhang, Yiyue
A1 Ruan, Hua
A1 Liu, Feng
A1 Zhu, Zuoyan
A1 Meng, Anming
A1 The ZAKOC Consortium
T1 Systematic genome editing of the genes on zebrafish Chromosome 1 by CRISPR/Cas9
JF Genome Research 
JO Genome Research 
YR 2020 
FD January 01 
VO 30 
IS 1 
SP 118 
OP 126 
DO 10.1101/gr.248559.119 
UL http://genome.cshlp.org/content/30/1/118.abstract 
AB Genome editing by the well-established CRISPR/Cas9 technology has greatly facilitated our understanding of many biological processes. However, a complete whole-genome knockout for any species or model organism has rarely been achieved. Here, we performed a systematic knockout of all the genes (1333) on Chromosome 1 in zebrafish, successfully mutated 1029 genes, and generated 1039 germline-transmissible alleles corresponding to 636 genes. Meanwhile, by high-throughput bioinformatics analysis, we found that sequence features play pivotal roles in effective gRNA targeting at specific genes of interest, while the success rate of gene targeting positively correlates with GC content of the target sites. Moreover, we found that nearly one-fourth of all mutants are related to human diseases, and several representative CRISPR/Cas9-generated mutants are described here. Furthermore, we tried to identify the underlying mechanisms leading to distinct phenotypes between genetic mutants and antisense morpholino-mediated knockdown embryos. Altogether, this work has generated the first chromosome-wide collection of zebrafish genetic mutants by the CRISPR/Cas9 technology, which will serve as a valuable resource for the community, and our bioinformatics analysis also provides some useful guidance to design gene-specific gRNAs for successful gene editing.