TY  - JOUR
A1  - Sun, Yonghua
A1  - Zhang, Bo
A1  - Luo, Lingfei
A1  - Shi, De-Li
A1  - Wang, Han
A1  - Cui, Zongbin
A1  - Huang, Honghui
A1  - Cao, Ying
A1  - Shu, Xiaodong
A1  - Zhang, Wenqing
A1  - Zhou, Jianfeng
A1  - Li, Yun
A1  - Du, Jiulin
A1  - Zhao, Qingshun
A1  - Chen, Jun
A1  - Zhong, Hanbing
A1  - Zhong, Tao P.
A1  - Li, Li
A1  - Xiong, Jing-Wei
A1  - Peng, Jinrong
A1  - Xiao, Wuhan
A1  - Zhang, Jian
A1  - Yao, Jihua
A1  - Yin, Zhan
A1  - Mo, Xianming
A1  - Peng, Gang
A1  - Zhu, Jun
A1  - Chen, Yan
A1  - Zhou, Yong
A1  - Liu, Dong
A1  - Pan, Weijun
A1  - Zhang, Yiyue
A1  - Ruan, Hua
A1  - Liu, Feng
A1  - Zhu, Zuoyan
A1  - Meng, Anming
A1  - The ZAKOC Consortium
T1  - Systematic genome editing of the genes on zebrafish Chromosome 1 by CRISPR/Cas9
Y1  - 2020/01/01 
JF  - Genome Research 
JO  - Genome Research 
SP  - 118 
EP  - 126 
DO  - 10.1101/gr.248559.119 
VL  - 30 
IS  - 1 
UR  - http://genome.cshlp.org/content/30/1/118.abstract 
N2  - Genome editing by the well-established CRISPR/Cas9 technology has greatly facilitated our understanding of many biological processes. However, a complete whole-genome knockout for any species or model organism has rarely been achieved. Here, we performed a systematic knockout of all the genes (1333) on Chromosome 1 in zebrafish, successfully mutated 1029 genes, and generated 1039 germline-transmissible alleles corresponding to 636 genes. Meanwhile, by high-throughput bioinformatics analysis, we found that sequence features play pivotal roles in effective gRNA targeting at specific genes of interest, while the success rate of gene targeting positively correlates with GC content of the target sites. Moreover, we found that nearly one-fourth of all mutants are related to human diseases, and several representative CRISPR/Cas9-generated mutants are described here. Furthermore, we tried to identify the underlying mechanisms leading to distinct phenotypes between genetic mutants and antisense morpholino-mediated knockdown embryos. Altogether, this work has generated the first chromosome-wide collection of zebrafish genetic mutants by the CRISPR/Cas9 technology, which will serve as a valuable resource for the community, and our bioinformatics analysis also provides some useful guidance to design gene-specific gRNAs for successful gene editing. 
ER  -