@article{Sun01012020,
author = {Sun, Yonghua and Zhang, Bo and Luo, Lingfei and Shi, De-Li and Wang, Han and Cui, Zongbin and Huang, Honghui and Cao, Ying and Shu, Xiaodong and Zhang, Wenqing and Zhou, Jianfeng and Li, Yun and Du, Jiulin and Zhao, Qingshun and Chen, Jun and Zhong, Hanbing and Zhong, Tao P. and Li, Li and Xiong, Jing-Wei and Peng, Jinrong and Xiao, Wuhan and Zhang, Jian and Yao, Jihua and Yin, Zhan and Mo, Xianming and Peng, Gang and Zhu, Jun and Chen, Yan and Zhou, Yong and Liu, Dong and Pan, Weijun and Zhang, Yiyue and Ruan, Hua and Liu, Feng and Zhu, Zuoyan and Meng, Anming and The ZAKOC Consortium}, 
title = {Systematic genome editing of the genes on zebrafish Chromosome 1 by CRISPR/Cas9},
volume = {30}, 
number = {1}, 
pages = {118-126}, 
year = {2020}, 
doi = {10.1101/gr.248559.119}, 
abstract ={Genome editing by the well-established CRISPR/Cas9 technology has greatly facilitated our understanding of many biological processes. However, a complete whole-genome knockout for any species or model organism has rarely been achieved. Here, we performed a systematic knockout of all the genes (1333) on Chromosome 1 in zebrafish, successfully mutated 1029 genes, and generated 1039 germline-transmissible alleles corresponding to 636 genes. Meanwhile, by high-throughput bioinformatics analysis, we found that sequence features play pivotal roles in effective gRNA targeting at specific genes of interest, while the success rate of gene targeting positively correlates with GC content of the target sites. Moreover, we found that nearly one-fourth of all mutants are related to human diseases, and several representative CRISPR/Cas9-generated mutants are described here. Furthermore, we tried to identify the underlying mechanisms leading to distinct phenotypes between genetic mutants and antisense morpholino-mediated knockdown embryos. Altogether, this work has generated the first chromosome-wide collection of zebrafish genetic mutants by the CRISPR/Cas9 technology, which will serve as a valuable resource for the community, and our bioinformatics analysis also provides some useful guidance to design gene-specific gRNAs for successful gene editing.}, 
URL = {http://genome.cshlp.org/content/30/1/118.abstract}, 
eprint = {http://genome.cshlp.org/content/30/1/118.full.pdf+html}, 
journal = {Genome Research} 
}