RT Journal
A1 Latorra, D
A1 Stern, C M
A1 Schanfield, M S
T1 Characterization of human AFLP systems apolipoprotein B, phenylalanine hydroxylase, and D1S80.
JF Genome Research 
JO Genome Research 
YR 1994 
FD June 01 
VO 3 
IS 6 
SP 351 
OP 358 
UL http://genome.cshlp.org/content/3/6/351.abstract 
AB Methodology is presented for amplified fragment length polymorphism (AFLP) typing using a nonisotopic, PCR protocol. Human variable number tandem repeat (VNTR) loci used for identification in forensic and paternity testing were optimized for reaction and thermal-cycling parameters. Loci analyzed were the apolipoprotein B (APOB) 3' hypervariable region (HVR), phenylalanine hydroxylase 3' HVR (PAH), and D1S80. Coamplification of a monomorphic beta-globin fragment serves as an amplification control. Biotin is integrated into PCR amplicon through primer incorporation. AFLP products undergo agarose gel electrophoresis and Southern transfer to a nylon membrane. Amplicons were detected using a streptavidin-enzyme conjugate. Either colorimetric- or chemiluminescent-developed bands are genotyped using locus-specific allele ladders with known VNTR repeat numbers. Using this methodology, we have successfully typed > 500 individuals from three population groups for each locus during data basing and casework.