TY  - JOUR
A1  - Latorra, D
A1  - Stern, C M
A1  - Schanfield, M S
T1  - Characterization of human AFLP systems apolipoprotein B, phenylalanine hydroxylase, and D1S80.
Y1  - 1994/06/01 
JF  - Genome Research 
JO  - Genome Research 
SP  - 351 
EP  - 358 
VL  - 3 
IS  - 6 
UR  - http://genome.cshlp.org/content/3/6/351.abstract 
N2  - Methodology is presented for amplified fragment length polymorphism (AFLP) typing using a nonisotopic, PCR protocol. Human variable number tandem repeat (VNTR) loci used for identification in forensic and paternity testing were optimized for reaction and thermal-cycling parameters. Loci analyzed were the apolipoprotein B (APOB) 3' hypervariable region (HVR), phenylalanine hydroxylase 3' HVR (PAH), and D1S80. Coamplification of a monomorphic beta-globin fragment serves as an amplification control. Biotin is integrated into PCR amplicon through primer incorporation. AFLP products undergo agarose gel electrophoresis and Southern transfer to a nylon membrane. Amplicons were detected using a streptavidin-enzyme conjugate. Either colorimetric- or chemiluminescent-developed bands are genotyped using locus-specific allele ladders with known VNTR repeat numbers. Using this methodology, we have successfully typed > 500 individuals from three population groups for each locus during data basing and casework. 
ER  -