@article{Latorra01061994,
author = {Latorra, D and Stern, C M and Schanfield, M S}, 
title = {Characterization of human AFLP systems apolipoprotein B, phenylalanine hydroxylase, and D1S80.},
volume = {3}, 
number = {6}, 
pages = {351-358}, 
year = {1994}, 
abstract ={Methodology is presented for amplified fragment length polymorphism (AFLP) typing using a nonisotopic, PCR protocol. Human variable number tandem repeat (VNTR) loci used for identification in forensic and paternity testing were optimized for reaction and thermal-cycling parameters. Loci analyzed were the apolipoprotein B (APOB) 3' hypervariable region (HVR), phenylalanine hydroxylase 3' HVR (PAH), and D1S80. Coamplification of a monomorphic beta-globin fragment serves as an amplification control. Biotin is integrated into PCR amplicon through primer incorporation. AFLP products undergo agarose gel electrophoresis and Southern transfer to a nylon membrane. Amplicons were detected using a streptavidin-enzyme conjugate. Either colorimetric- or chemiluminescent-developed bands are genotyped using locus-specific allele ladders with known VNTR repeat numbers. Using this methodology, we have successfully typed > 500 individuals from three population groups for each locus during data basing and casework.}, 
URL = {http://genome.cshlp.org/content/3/6/351.abstract}, 
eprint = {http://genome.cshlp.org/content/3/6/351.full.pdf+html}, 
journal = {Genome Research} 
}