RT Journal
A1 Costa, G L
A1 Grafsky, A
A1 Weiner, M P
T1 Cloning and analysis of PCR-generated DNA fragments.
JF Genome Research 
JO Genome Research 
YR 1994 
FD June 01 
VO 3 
IS 6 
SP 338 
OP 345 
UL http://genome.cshlp.org/content/3/6/338.abstract 
AB Methods are presented for the improved yield and analysis of blunt-ended cloning of PCR-generated DNA fragments. We show that Pfu DNA polymerase polishing of Taq DNA polymerase-generated fragments increases the yield and efficiency of cloning. Using a triple primer set consisting of two outside, asymmetrically distanced primers and one fragment-specific primer, both the presence and orientation of cloned inserts can be determined. Application of these methods allows the generation and cloning of a fragment in 1 day and the analysis of putative clones the next, thereby saving a substantial amount of both time and effort.