TY  - JOUR
A1  - Costa, G L
A1  - Grafsky, A
A1  - Weiner, M P
T1  - Cloning and analysis of PCR-generated DNA fragments.
Y1  - 1994/06/01 
JF  - Genome Research 
JO  - Genome Research 
SP  - 338 
EP  - 345 
VL  - 3 
IS  - 6 
UR  - http://genome.cshlp.org/content/3/6/338.abstract 
N2  - Methods are presented for the improved yield and analysis of blunt-ended cloning of PCR-generated DNA fragments. We show that Pfu DNA polymerase polishing of Taq DNA polymerase-generated fragments increases the yield and efficiency of cloning. Using a triple primer set consisting of two outside, asymmetrically distanced primers and one fragment-specific primer, both the presence and orientation of cloned inserts can be determined. Application of these methods allows the generation and cloning of a fragment in 1 day and the analysis of putative clones the next, thereby saving a substantial amount of both time and effort. 
ER  -