@article{Costa01061994,
author = {Costa, G L and Grafsky, A and Weiner, M P}, 
title = {Cloning and analysis of PCR-generated DNA fragments.},
volume = {3}, 
number = {6}, 
pages = {338-345}, 
year = {1994}, 
abstract ={Methods are presented for the improved yield and analysis of blunt-ended cloning of PCR-generated DNA fragments. We show that Pfu DNA polymerase polishing of Taq DNA polymerase-generated fragments increases the yield and efficiency of cloning. Using a triple primer set consisting of two outside, asymmetrically distanced primers and one fragment-specific primer, both the presence and orientation of cloned inserts can be determined. Application of these methods allows the generation and cloning of a fragment in 1 day and the analysis of putative clones the next, thereby saving a substantial amount of both time and effort.}, 
URL = {http://genome.cshlp.org/content/3/6/338.abstract}, 
eprint = {http://genome.cshlp.org/content/3/6/338.full.pdf+html}, 
journal = {Genome Research} 
}