TY  - JOUR
A1  - Zhang, Shilin
A1  - Wang, Yichen
A1  - Jia, Lin
A1  - Wen, Xue
A1  - Du, Zhonghua
A1  - Wang, Cong
A1  - Hao, Yajing
A1  - Yu, Dehai
A1  - Zhou, Lei
A1  - Chen, Naifei
A1  - Chen, Jingcheng
A1  - Chen, Huiling
A1  - Zhang, Hui
A1  - Celik, Ilkay
A1  - Gülsoy, Günhan
A1  - Luo, Jianjun
A1  - Qin, Baoming
A1  - Cui, Xueling
A1  - Liu, Zhonghui
A1  - Zhang, Songling
A1  - Esteban, Miguel A.
A1  - Ay, Ferhat
A1  - Xu, Wei
A1  - Chen, Runsheng
A1  - Li, Wei
A1  - Hoffman, Andrew R.
A1  - Hu, Ji-Fan
A1  - Cui, Jiuwei
T1  - Profiling the long noncoding RNA interaction network in the regulatory elements of target genes by chromatin in situ reverse transcription sequencing
Y1  - 2019/09/01 
JF  - Genome Research 
JO  - Genome Research 
SP  - 1521 
EP  - 1532 
DO  - 10.1101/gr.244996.118 
VL  - 29 
IS  - 9 
UR  - http://genome.cshlp.org/content/29/9/1521.abstract 
N2  - Long noncoding RNAs (lncRNAs) can regulate the activity of target genes by participating in the organization of chromatin architecture. We have devised a “chromatin-RNA in situ reverse transcription sequencing” (CRIST-seq) approach to profile the lncRNA interaction network in gene regulatory elements by combining the simplicity of RNA biotin labeling with the specificity of the CRISPR/Cas9 system. Using gene-specific gRNAs, we describe a pluripotency-specific lncRNA interacting network in the promoters of Sox2 and Pou5f1, two critical stem cell factors that are required for the maintenance of pluripotency. The promoter-interacting lncRNAs were specifically activated during reprogramming into pluripotency. Knockdown of these lncRNAs caused the stem cells to exit from pluripotency. In contrast, overexpression of the pluripotency-associated lncRNA activated the promoters of core stem cell factor genes and enhanced fibroblast reprogramming into pluripotency. These CRIST-seq data suggest that the Sox2 and Pou5f1 promoters are organized within a unique lncRNA interaction network that determines the fate of pluripotency during reprogramming. This CRIST approach may be broadly used to map lncRNA interaction networks at target loci across the genome. 
ER  -