RT Journal
A1 Yeh, Chung-Shu
A1 Wang, Zhifeng
A1 Miao, Fang
A1 Ma, Hongyan
A1 Kao, Chung-Ting
A1 Hsu, Tzu-Shu
A1 Yu, Jhong-He
A1 Hung, Er-Tsi
A1 Lin, Chia-Chang
A1 Kuan, Chen-Yu
A1 Tsai, Ni-Chiao
A1 Zhou, Chenguang
A1 Qu, Guan-Zheng
A1 Jiang, Jing
A1 Liu, Guifeng
A1 Wang, Jack P.
A1 Li, Wei
A1 Chiang, Vincent L.
A1 Chang, Tien-Hsien
A1 Lin, Ying-Chung Jimmy
T1 A novel synthetic-genetic-array–based yeast one-hybrid system for high discovery rate and short processing time
JF Genome Research 
JO Genome Research 
YR 2019 
FD August 01 
VO 29 
IS 8 
SP 1343 
OP 1351 
DO 10.1101/gr.245951.118 
UL http://genome.cshlp.org/content/29/8/1343.abstract 
AB Eukaryotic gene expression is often tightly regulated by interactions between transcription factors (TFs) and their DNA cis targets. Yeast one-hybrid (Y1H) is one of the most extensively used methods to discover these interactions. We developed a high-throughput meiosis-directed yeast one-hybrid system using the Magic Markers of the synthetic genetic array analysis. The system has a transcription factor–DNA interaction discovery rate twice as high as the conventional diploid-mating approach and a processing time nearly one-tenth of the haploid-transformation method. The system also offers the highest accuracy in identifying TF–DNA interactions that can be authenticated in vivo by chromatin immunoprecipitation. With these unique features, this meiosis-directed Y1H system is particularly suited for constructing novel and comprehensive genome-scale gene regulatory networks for various organisms.