TY  - JOUR
A1  - Yeh, Chung-Shu
A1  - Wang, Zhifeng
A1  - Miao, Fang
A1  - Ma, Hongyan
A1  - Kao, Chung-Ting
A1  - Hsu, Tzu-Shu
A1  - Yu, Jhong-He
A1  - Hung, Er-Tsi
A1  - Lin, Chia-Chang
A1  - Kuan, Chen-Yu
A1  - Tsai, Ni-Chiao
A1  - Zhou, Chenguang
A1  - Qu, Guan-Zheng
A1  - Jiang, Jing
A1  - Liu, Guifeng
A1  - Wang, Jack P.
A1  - Li, Wei
A1  - Chiang, Vincent L.
A1  - Chang, Tien-Hsien
A1  - Lin, Ying-Chung Jimmy
T1  - A novel synthetic-genetic-array–based yeast one-hybrid system for high discovery rate and short processing time
Y1  - 2019/08/01 
JF  - Genome Research 
JO  - Genome Research 
SP  - 1343 
EP  - 1351 
DO  - 10.1101/gr.245951.118 
VL  - 29 
IS  - 8 
UR  - http://genome.cshlp.org/content/29/8/1343.abstract 
N2  - Eukaryotic gene expression is often tightly regulated by interactions between transcription factors (TFs) and their DNA cis targets. Yeast one-hybrid (Y1H) is one of the most extensively used methods to discover these interactions. We developed a high-throughput meiosis-directed yeast one-hybrid system using the Magic Markers of the synthetic genetic array analysis. The system has a transcription factor–DNA interaction discovery rate twice as high as the conventional diploid-mating approach and a processing time nearly one-tenth of the haploid-transformation method. The system also offers the highest accuracy in identifying TF–DNA interactions that can be authenticated in vivo by chromatin immunoprecipitation. With these unique features, this meiosis-directed Y1H system is particularly suited for constructing novel and comprehensive genome-scale gene regulatory networks for various organisms. 
ER  -