@article{Yeh01082019,
author = {Yeh, Chung-Shu and Wang, Zhifeng and Miao, Fang and Ma, Hongyan and Kao, Chung-Ting and Hsu, Tzu-Shu and Yu, Jhong-He and Hung, Er-Tsi and Lin, Chia-Chang and Kuan, Chen-Yu and Tsai, Ni-Chiao and Zhou, Chenguang and Qu, Guan-Zheng and Jiang, Jing and Liu, Guifeng and Wang, Jack P. and Li, Wei and Chiang, Vincent L. and Chang, Tien-Hsien and Lin, Ying-Chung Jimmy}, 
title = {A novel synthetic-genetic-array–based yeast one-hybrid system for high discovery rate and short processing time},
volume = {29}, 
number = {8}, 
pages = {1343-1351}, 
year = {2019}, 
doi = {10.1101/gr.245951.118}, 
abstract ={Eukaryotic gene expression is often tightly regulated by interactions between transcription factors (TFs) and their DNA cis targets. Yeast one-hybrid (Y1H) is one of the most extensively used methods to discover these interactions. We developed a high-throughput meiosis-directed yeast one-hybrid system using the Magic Markers of the synthetic genetic array analysis. The system has a transcription factor–DNA interaction discovery rate twice as high as the conventional diploid-mating approach and a processing time nearly one-tenth of the haploid-transformation method. The system also offers the highest accuracy in identifying TF–DNA interactions that can be authenticated in vivo by chromatin immunoprecipitation. With these unique features, this meiosis-directed Y1H system is particularly suited for constructing novel and comprehensive genome-scale gene regulatory networks for various organisms.}, 
URL = {http://genome.cshlp.org/content/29/8/1343.abstract}, 
eprint = {http://genome.cshlp.org/content/29/8/1343.full.pdf+html}, 
journal = {Genome Research} 
}