TY  - JOUR
A1  - Labun, Kornel
A1  - Guo, Xiaoge
A1  - Chavez, Alejandro
A1  - Church, George
A1  - Gagnon, James A.
A1  - Valen, Eivind
T1  - Accurate analysis of genuine CRISPR editing events with ampliCan
Y1  - 2019/05/01 
JF  - Genome Research 
JO  - Genome Research 
SP  - 843 
EP  - 847 
DO  - 10.1101/gr.244293.118 
VL  - 29 
IS  - 5 
UR  - http://genome.cshlp.org/content/29/5/843.abstract 
N2  - We present ampliCan, an analysis tool for genome editing that unites highly precise quantification and visualization of genuine genome editing events. ampliCan features nuclease-optimized alignments, filtering of experimental artifacts, event-specific normalization, and off-target read detection and quantifies insertions, deletions, HDR repair, as well as targeted base editing. It is scalable to thousands of amplicon sequencing–based experiments from any genome editing experiment, including CRISPR. It enables automated integration of controls and accounts for biases at every step of the analysis. We benchmarked ampliCan on both real and simulated data sets against other leading tools, demonstrating that it outperformed all in the face of common confounding factors. 
ER  -