TY  - JOUR
A1  - Wang, Ou
A1  - Chin, Robert
A1  - Cheng, Xiaofang
A1  - Wu, Michelle Ka Yan
A1  - Mao, Qing
A1  - Tang, Jingbo
A1  - Sun, Yuhui
A1  - Anderson, Ellis
A1  - Lam, Han K.
A1  - Chen, Dan
A1  - Zhou, Yujun
A1  - Wang, Linying
A1  - Fan, Fei
A1  - Zou, Yan
A1  - Xie, Yinlong
A1  - Zhang, Rebecca Yu
A1  - Drmanac, Snezana
A1  - Nguyen, Darlene
A1  - Xu, Chongjun
A1  - Villarosa, Christian
A1  - Gablenz, Scott
A1  - Barua, Nina
A1  - Nguyen, Staci
A1  - Tian, Wenlan
A1  - Liu, Jia Sophie
A1  - Wang, Jingwan
A1  - Liu, Xiao
A1  - Qi, Xiaojuan
A1  - Chen, Ao
A1  - Wang, He
A1  - Dong, Yuliang
A1  - Zhang, Wenwei
A1  - Alexeev, Andrei
A1  - Yang, Huanming
A1  - Wang, Jian
A1  - Kristiansen, Karsten
A1  - Xu, Xun
A1  - Drmanac, Radoje
A1  - Peters, Brock A.
T1  - Efficient and unique cobarcoding of second-generation sequencing reads from long DNA molecules enabling cost-effective and accurate sequencing, haplotyping, and de novo assembly
Y1  - 2019/05/01 
JF  - Genome Research 
JO  - Genome Research 
SP  - 798 
EP  - 808 
DO  - 10.1101/gr.245126.118 
VL  - 29 
IS  - 5 
UR  - http://genome.cshlp.org/content/29/5/798.abstract 
N2  - Here, we describe single-tube long fragment read (stLFR), a technology that enables sequencing of data from long DNA molecules using economical second-generation sequencing technology. It is based on adding the same barcode sequence to subfragments of the original long DNA molecule (DNA cobarcoding). To achieve this efficiently, stLFR uses the surface of microbeads to create millions of miniaturized barcoding reactions in a single tube. Using a combinatorial process, up to 3.6 billion unique barcode sequences were generated on beads, enabling practically nonredundant cobarcoding with 50 million barcodes per sample. Using stLFR, we demonstrate efficient unique cobarcoding of more than 8 million 20- to 300-kb genomic DNA fragments. Analysis of the human genome NA12878 with stLFR demonstrated high-quality variant calling and phase block lengths up to N50 34 Mb. We also demonstrate detection of complex structural variants and complete diploid de novo assembly of NA12878. These analyses were all performed using single stLFR libraries, and their construction did not significantly add to the time or cost of whole-genome sequencing (WGS) library preparation. stLFR represents an easily automatable solution that enables high-quality sequencing, phasing, SV detection, scaffolding, cost-effective diploid de novo genome assembly, and other long DNA sequencing applications. 
ER  -