@article{Wang01052019,
author = {Wang, Ou and Chin, Robert and Cheng, Xiaofang and Wu, Michelle Ka Yan and Mao, Qing and Tang, Jingbo and Sun, Yuhui and Anderson, Ellis and Lam, Han K. and Chen, Dan and Zhou, Yujun and Wang, Linying and Fan, Fei and Zou, Yan and Xie, Yinlong and Zhang, Rebecca Yu and Drmanac, Snezana and Nguyen, Darlene and Xu, Chongjun and Villarosa, Christian and Gablenz, Scott and Barua, Nina and Nguyen, Staci and Tian, Wenlan and Liu, Jia Sophie and Wang, Jingwan and Liu, Xiao and Qi, Xiaojuan and Chen, Ao and Wang, He and Dong, Yuliang and Zhang, Wenwei and Alexeev, Andrei and Yang, Huanming and Wang, Jian and Kristiansen, Karsten and Xu, Xun and Drmanac, Radoje and Peters, Brock A.}, 
title = {Efficient and unique cobarcoding of second-generation sequencing reads from long DNA molecules enabling cost-effective and accurate sequencing, haplotyping, and de novo assembly},
volume = {29}, 
number = {5}, 
pages = {798-808}, 
year = {2019}, 
doi = {10.1101/gr.245126.118}, 
abstract ={Here, we describe single-tube long fragment read (stLFR), a technology that enables sequencing of data from long DNA molecules using economical second-generation sequencing technology. It is based on adding the same barcode sequence to subfragments of the original long DNA molecule (DNA cobarcoding). To achieve this efficiently, stLFR uses the surface of microbeads to create millions of miniaturized barcoding reactions in a single tube. Using a combinatorial process, up to 3.6 billion unique barcode sequences were generated on beads, enabling practically nonredundant cobarcoding with 50 million barcodes per sample. Using stLFR, we demonstrate efficient unique cobarcoding of more than 8 million 20- to 300-kb genomic DNA fragments. Analysis of the human genome NA12878 with stLFR demonstrated high-quality variant calling and phase block lengths up to N50 34 Mb. We also demonstrate detection of complex structural variants and complete diploid de novo assembly of NA12878. These analyses were all performed using single stLFR libraries, and their construction did not significantly add to the time or cost of whole-genome sequencing (WGS) library preparation. stLFR represents an easily automatable solution that enables high-quality sequencing, phasing, SV detection, scaffolding, cost-effective diploid de novo genome assembly, and other long DNA sequencing applications.}, 
URL = {http://genome.cshlp.org/content/29/5/798.abstract}, 
eprint = {http://genome.cshlp.org/content/29/5/798.full.pdf+html}, 
journal = {Genome Research} 
}