TY  - JOUR
A1  - Wang, Shiyan
A1  - Chen, Jiahuan
A1  - Garcia, Sara P.
A1  - Liang, Xiaodong
A1  - Zhang, Fang
A1  - Yan, Pengyi
A1  - Yu, Huijing
A1  - Wei, Weiting
A1  - Li, Zixuan
A1  - Wang, Jingfang
A1  - Le, Huangying
A1  - Han, Zeguang
A1  - Luo, Xusheng
A1  - Day, Daniel S.
A1  - Stevens, Sean M.
A1  - Zhang, Yan
A1  - Park, Peter J.
A1  - Liu, Zhi-jie
A1  - Sun, Kun
A1  - Yuan, Guo-cheng
A1  - Pu, William T.
A1  - Zhang, Bing
T1  - A dynamic and integrated epigenetic program at distal regions orchestrates transcriptional responses to VEGFA
Y1  - 2019/02/01 
JF  - Genome Research 
JO  - Genome Research 
SP  - 193 
EP  - 207 
DO  - 10.1101/gr.239053.118 
VL  - 29 
IS  - 2 
UR  - http://genome.cshlp.org/content/29/2/193.abstract 
N2  - Cell behaviors are dictated by epigenetic and transcriptional programs. Little is known about how extracellular stimuli modulate these programs to reshape gene expression and control cell behavioral responses. Here, we interrogated the epigenetic and transcriptional response of endothelial cells to VEGFA treatment and found rapid chromatin changes that mediate broad transcriptomic alterations. VEGFA-responsive genes were associated with active promoters, but changes in promoter histone marks were not tightly linked to gene expression changes. VEGFA altered transcription factor occupancy and the distal epigenetic landscape, which profoundly contributed to VEGFA-dependent changes in gene expression. Integration of gene expression, dynamic enhancer, and transcription factor occupancy changes induced by VEGFA yielded a VEGFA-regulated transcriptional regulatory network, which revealed that the small MAF transcription factors are master regulators of the VEGFA transcriptional program and angiogenesis. Collectively these results revealed that extracellular stimuli rapidly reconfigure the chromatin landscape to coordinately regulate biological responses. 
ER  -