TY  - JOUR
A1  - van Eijk, Patrick
A1  - Nandi, Shuvro Prokash
A1  - Yu, Shirong
A1  - Bennett, Mark
A1  - Leadbitter, Matthew
A1  - Teng, Yumin
A1  - Reed, Simon H.
T1  - Nucleosome remodeling at origins of global genome–nucleotide excision repair occurs at the boundaries of higher-order chromatin structure
Y1  - 2019/01/01 
JF  - Genome Research 
JO  - Genome Research 
SP  - 74 
EP  - 84 
DO  - 10.1101/gr.237198.118 
VL  - 29 
IS  - 1 
UR  - http://genome.cshlp.org/content/29/1/74.abstract 
N2  - Repair of UV-induced DNA damage requires chromatin remodeling. How repair is initiated in chromatin remains largely unknown. We recently demonstrated that global genome–nucleotide excision repair (GG-NER) in chromatin is organized into domains in relation to open reading frames. Here, we define these domains, identifying the genomic locations from which repair is initiated. By examining DNA damage–induced changes in the linear structure of nucleosomes at these sites, we demonstrate how chromatin remodeling is initiated during GG-NER. In undamaged cells, we show that the GG-NER complex occupies chromatin, establishing the nucleosome structure at these genomic locations, which we refer to as GG-NER complex binding sites (GCBSs). We demonstrate that these sites are frequently located at genomic boundaries that delineate chromosomally interacting domains (CIDs). These boundaries define domains of higher-order nucleosome–nucleosome interaction. We demonstrate that initiation of GG-NER in chromatin is accompanied by the disruption of dynamic nucleosomes that flank GCBSs by the GG-NER complex. 
ER  -