TY  - JOUR
A1  - Wang, Isabel X.
A1  - Grunseich, Christopher
A1  - Fox, Jennifer
A1  - Burdick, Joshua
A1  - Zhu, Zhengwei
A1  - Ravazian, Niema
A1  - Hafner, Markus
A1  - Cheung, Vivian G.
T1  - Human proteins that interact with RNA/DNA hybrids
Y1  - 2018/09/01 
JF  - Genome Research 
JO  - Genome Research 
SP  - 1405 
EP  - 1414 
DO  - 10.1101/gr.237362.118 
VL  - 28 
IS  - 9 
UR  - http://genome.cshlp.org/content/28/9/1405.abstract 
N2  - RNA/DNA hybrids form when RNA hybridizes with its template DNA generating a three-stranded structure known as the R-loop. Knowledge of how they form and resolve, as well as their functional roles, is limited. Here, by pull-down assays followed by mass spectrometry, we identified 803 proteins that bind to RNA/DNA hybrids. Because these proteins were identified using in vitro assays, we confirmed that they bind to R-loops in vivo. They include proteins that are involved in a variety of functions, including most steps of RNA processing. The proteins are enriched for K homology (KH) and helicase domains. Among them, more than 300 proteins preferred binding to hybrids than double-stranded DNA. These proteins serve as starting points for mechanistic studies to elucidate what RNA/DNA hybrids regulate and how they are regulated. 
ER  -