TY  - JOUR
A1  - Pellegrino, Maurizio
A1  - Sciambi, Adam
A1  - Treusch, Sebastian
A1  - Durruthy-Durruthy, Robert
A1  - Gokhale, Kaustubh
A1  - Jacob, Jose
A1  - Chen, Tina X.
A1  - Geis, Jennifer A.
A1  - Oldham, William
A1  - Matthews, Jairo
A1  - Kantarjian, Hagop
A1  - Futreal, P. Andrew
A1  - Patel, Keyur
A1  - Jones, Keith W.
A1  - Takahashi, Koichi
A1  - Eastburn, Dennis J.
T1  - High-throughput single-cell DNA sequencing of acute myeloid leukemia tumors with droplet microfluidics
Y1  - 2018/09/01 
JF  - Genome Research 
JO  - Genome Research 
SP  - 1345 
EP  - 1352 
DO  - 10.1101/gr.232272.117 
VL  - 28 
IS  - 9 
UR  - http://genome.cshlp.org/content/28/9/1345.abstract 
N2  - To enable the characterization of genetic heterogeneity in tumor cell populations, we developed a novel microfluidic approach that barcodes amplified genomic DNA from thousands of individual cancer cells confined to droplets. The barcodes are then used to reassemble the genetic profiles of cells from next-generation sequencing data. By using this approach, we sequenced longitudinally collected acute myeloid leukemia (AML) tumor populations from two patients and genotyped up to 62 disease relevant loci across more than 16,000 individual cells. Targeted single-cell sequencing was able to sensitively identify cells harboring pathogenic mutations during complete remission and uncovered complex clonal evolution within AML tumors that was not observable with bulk sequencing. We anticipate that this approach will make feasible the routine analysis of AML heterogeneity, leading to improved stratification and therapy selection for the disease. 
ER  -