RT Journal
A1 Soudet, Julien
A1 Gill, Jatinder Kaur
A1 Stutz, Françoise
T1 Noncoding transcription influences the replication initiation program through chromatin regulation
JF Genome Research 
JO Genome Research 
YR 2018 
FD December 01 
VO 28 
IS 12 
SP 1882 
OP 1893 
DO 10.1101/gr.239582.118 
UL http://genome.cshlp.org/content/28/12/1882.abstract 
AB In eukaryotic organisms, replication initiation follows a temporal program. Among the parameters that regulate this program in Saccharomyces cerevisiae, chromatin structure has been at the center of attention without considering the contribution of transcription. Here, we revisit the replication initiation program in the light of widespread genomic noncoding transcription. We find that noncoding RNA transcription termination in the vicinity of autonomously replicating sequences (ARSs) shields replication initiation from transcriptional readthrough. Consistently, high natural nascent transcription correlates with low ARS efficiency and late replication timing. High readthrough transcription is also linked to increased nucleosome occupancy and high levels of H3K36me3. Moreover, forcing ARS readthrough transcription promotes these chromatin features. Finally, replication initiation defects induced by increased transcriptional readthrough are partially rescued in the absence of H3K36 methylation. Altogether, these observations indicate that natural noncoding transcription into ARSs influences replication initiation through chromatin regulation.