TY  - JOUR
A1  - Baudement, Marie-Odile
A1  - Cournac, Axel
A1  - Court, Franck
A1  - Seveno, Marie
A1  - Parrinello, Hugues
A1  - Reynes, Christelle
A1  - Sabatier, Robert
A1  - Bouschet, Tristan
A1  - Yi, Zhou
A1  - Sallis, Sephora
A1  - Tancelin, Mathilde
A1  - Rebouissou, Cosette
A1  - Cathala, Guy
A1  - Lesne, Annick
A1  - Mozziconacci, Julien
A1  - Journot, Laurent
A1  - Forné, Thierry
T1  - High-salt–recovered sequences are associated with the active chromosomal compartment and with large ribonucleoprotein complexes including nuclear bodies
Y1  - 2018/11/01 
JF  - Genome Research 
JO  - Genome Research 
SP  - 1733 
EP  - 1746 
DO  - 10.1101/gr.237073.118 
VL  - 28 
IS  - 11 
UR  - http://genome.cshlp.org/content/28/11/1733.abstract 
N2  - The mammalian cell nucleus contains numerous discrete suborganelles named nuclear bodies. While recruitment of specific genomic regions into these large ribonucleoprotein (RNP) complexes critically contributes to higher-order functional chromatin organization, such regions remain ill-defined. We have developed the high-salt–recovered sequences-sequencing (HRS-seq) method, a straightforward genome-wide approach whereby we isolated and sequenced genomic regions associated with large high-salt insoluble RNP complexes. By using mouse embryonic stem cells (ESCs), we showed that these regions essentially correspond to the most highly expressed genes, and to cis-regulatory sequences like super-enhancers, that belong to the active A chromosomal compartment. They include both cell-type–specific genes, such as pluripotency genes in ESCs, and housekeeping genes associated with nuclear bodies, such as histone and snRNA genes that are central components of Histone Locus Bodies and Cajal bodies. We conclude that HRSs are associated with the active chromosomal compartment and with large RNP complexes including nuclear bodies. Association of such chromosomal regions with nuclear bodies is in agreement with the recently proposed phase separation model for transcription control and might thus play a central role in organizing the active chromosomal compartment in mammals. 
ER  -