RT Journal
A1 Fu, Yonggui
A1 Chen, Liutao
A1 Chen, Chengyong
A1 Ge, Yutong
A1 Kang, Mingjing
A1 Song, Zili
A1 Li, Jingwen
A1 Feng, Yuchao
A1 Huo, Zhanfeng
A1 He, Guopei
A1 Hou, Mengmeng
A1 Chen, Shangwu
A1 Xu, Anlong
T1 Crosstalk between alternative polyadenylation and miRNAs in the regulation of protein translational efficiency
JF Genome Research 
JO Genome Research 
YR 2018 
FD November 01 
VO 28 
IS 11 
SP 1656 
OP 1663 
DO 10.1101/gr.231506.117 
UL http://genome.cshlp.org/content/28/11/1656.abstract 
AB 3′ UTRs play important roles in the gene regulation network via their influence on mRNA stability, translational efficiency, and subcellular localization. For a given gene, 3′ UTRs of different lengths generated by alternative polyadenylation (APA) may result in functional differences in regulation. The mechanistic details of how length changes of 3′ UTRs alter gene function remain unclear. By combining APA sequencing and polysome profiling, we observed that mRNA isoforms with shorter 3′ UTRs were bound with more polysomes in six cell lines but not in NIH3T3 cells, suggesting that changing 3′ UTRs to shorter isoforms may lead to a higher gene translational efficiency. By interfering with the expression of TNRC6A and analyzing AGO2-PAR-CLIP data, we revealed that the APA effect on translational efficiency was mainly regulated by miRNAs, and this regulation was cell cycle dependent. The discrepancy between NIH3T3 and other cell lines was due to contact inhibition of NIH3T3. Thus, the crosstalk between APA and miRNAs may be needed for the regulation of protein translational efficiency.