TY  - JOUR
A1  - Gao, Yan
A1  - Ni, Xiaohui
A1  - Guo, Hua
A1  - Su, Zhe
A1  - Ba, Yi
A1  - Tong, Zhongsheng
A1  - Guo, Zhi
A1  - Yao, Xin
A1  - Chen, Xixi
A1  - Yin, Jian
A1  - Yan, Zhao
A1  - Guo, Lin
A1  - Liu, Ying
A1  - Bai, Fan
A1  - Xie, X. Sunney
A1  - Zhang, Ning
T1  - Single-cell sequencing deciphers a convergent evolution of copy number alterations from primary to circulating tumor cells
Y1  - 2017/08/01 
JF  - Genome Research 
JO  - Genome Research 
SP  - 1312 
EP  - 1322 
DO  - 10.1101/gr.216788.116 
VL  - 27 
IS  - 8 
UR  - http://genome.cshlp.org/content/27/8/1312.abstract 
N2  - Copy number alteration (CNA) is a major contributor to genome instability, a hallmark of cancer. Here, we studied genomic alterations in single primary tumor cells and circulating tumor cells (CTCs) from the same patient. Single-nucleotide variants (SNVs) in single cells from both samples occurred sporadically, whereas CNAs among primary tumor cells emerged accumulatively rather than abruptly, converging toward the CNA in CTCs. Focal CNAs affecting the MYC gene and the PTEN gene were observed only in a minor portion of primary tumor cells but were present in all CTCs, suggesting a strong selection toward metastasis. Single-cell structural variant (SV) analyses revealed a two-step mechanism, a complex rearrangement followed by gene amplification, for the simultaneous formation of anomalous CNAs in multiple chromosome regions. Integrative CNA analyses of 97 CTCs from 23 patients confirmed the convergence of CNAs and revealed single, concurrent, and mutually exclusive CNAs that could be the driving events in cancer metastasis. 
ER  -