RT Journal
A1 Clavijo, Bernardo J.
A1 Venturini, Luca
A1 Schudoma, Christian
A1 Accinelli, Gonzalo Garcia
A1 Kaithakottil, Gemy
A1 Wright, Jonathan
A1 Borrill, Philippa
A1 Kettleborough, George
A1 Heavens, Darren
A1 Chapman, Helen
A1 Lipscombe, James
A1 Barker, Tom
A1 Lu, Fu-Hao
A1 McKenzie, Neil
A1 Raats, Dina
A1 Ramirez-Gonzalez, Ricardo H.
A1 Coince, Aurore
A1 Peel, Ned
A1 Percival-Alwyn, Lawrence
A1 Duncan, Owen
A1 Trösch, Josua
A1 Yu, Guotai
A1 Bolser, Dan M.
A1 Namaati, Guy
A1 Kerhornou, Arnaud
A1 Spannagl, Manuel
A1 Gundlach, Heidrun
A1 Haberer, Georg
A1 Davey, Robert P.
A1 Fosker, Christine
A1 Palma, Federica Di
A1 Phillips, Andrew L.
A1 Millar, A. Harvey
A1 Kersey, Paul J.
A1 Uauy, Cristobal
A1 Krasileva, Ksenia V.
A1 Swarbreck, David
A1 Bevan, Michael W.
A1 Clark, Matthew D.
T1 An improved assembly and annotation of the allohexaploid wheat genome identifies complete families of agronomic genes and provides genomic evidence for chromosomal translocations
JF Genome Research 
JO Genome Research 
YR 2017 
FD May 01 
VO 27 
IS 5 
SP 885 
OP 896 
DO 10.1101/gr.217117.116 
UL http://genome.cshlp.org/content/27/5/885.abstract 
AB Advances in genome sequencing and assembly technologies are generating many high-quality genome sequences, but assemblies of large, repeat-rich polyploid genomes, such as that of bread wheat, remain fragmented and incomplete. We have generated a new wheat whole-genome shotgun sequence assembly using a combination of optimized data types and an assembly algorithm designed to deal with large and complex genomes. The new assembly represents >78% of the genome with a scaffold N50 of 88.8 kb that has a high fidelity to the input data. Our new annotation combines strand-specific Illumina RNA-seq and Pacific Biosciences (PacBio) full-length cDNAs to identify 104,091 high-confidence protein-coding genes and 10,156 noncoding RNA genes. We confirmed three known and identified one novel genome rearrangements. Our approach enables the rapid and scalable assembly of wheat genomes, the identification of structural variants, and the definition of complete gene models, all powerful resources for trait analysis and breeding of this key global crop.