RT Journal
A1 Vaser, Robert
A1 Sović, Ivan
A1 Nagarajan, Niranjan
A1 Šikić, Mile
T1 Fast and accurate de novo genome assembly from long uncorrected reads
JF Genome Research 
JO Genome Research 
YR 2017 
FD May 01 
VO 27 
IS 5 
SP 737 
OP 746 
DO 10.1101/gr.214270.116 
UL http://genome.cshlp.org/content/27/5/737.abstract 
AB The assembly of long reads from Pacific Biosciences and Oxford Nanopore Technologies typically requires resource-intensive error-correction and consensus-generation steps to obtain high-quality assemblies. We show that the error-correction step can be omitted and that high-quality consensus sequences can be generated efficiently with a SIMD-accelerated, partial-order alignment–based, stand-alone consensus module called Racon. Based on tests with PacBio and Oxford Nanopore data sets, we show that Racon coupled with miniasm enables consensus genomes with similar or better quality than state-of-the-art methods while being an order of magnitude faster.